PCR Master Mix Calculator (Method 1)

Enter your reaction parameters below for instant PCR master mix formulation.

Reaction Parameters

Number of Reactions:

Reaction Volume (µL):

Overage Percentage (%):

Reagent Concentrations

Taq Polymerase (U/µL):

Final Taq Concentration (U/rxn):

dNTP Mix Stock (mM):

Final dNTP Concentration (mM):

Primer Concentration (µM):

Final Primer Concentration (µM):

MgCl₂ Stock (mM):

Final MgCl₂ Concentration (mM):

Buffer Concentration:

Template DNA Volume (µL/rxn):

Master Mix Formulation

Total Reactions: 20

Total Master Mix Volume: 550 µL

Component	Volume per Reaction (µL)	Total Volume (µL)
Nuclease-free Water	-	-
PCR Buffer (X)	-	-
dNTP Mix	-	-
Forward Primer	-	-
Reverse Primer	-	-
MgCl₂	-	-
Taq Polymerase	-	-
Master Mix Total	-	-
Template DNA	2.0	40

Preparation Protocol:

Thaw all reagents on ice
Prepare master mix in a nuclease-free tube
Add components in the following order: Water → Buffer → dNTPs → Primers → MgCl₂ → Polymerase
Mix gently by pipetting, avoid bubbles
Aliquot - µL master mix per reaction tube
Add template DNA to each tube
Briefly centrifuge before placing in thermocycler

PCR Master Mix (Method 2)

Number of Reactions*:

Reaction Volume (µL):

Overage %:

Taq (U/µL):

Final Taq (U/rxn):

dNTP Stock (mM):

Final dNTP (mM):

Primer Conc. (µM):

Final Primer (µM):

MgCl₂ Stock (mM):

Final MgCl₂ (mM):

Buffer Conc (X):

Template DNA (µL/rxn):

Please enter a valid number of reactions (minimum 1).

Recommended PCR Products:

Taq Polymerase Kits • dNTP Mixes • PCR Buffers • MgCl₂ Solutions

What is PCR?

Polymerase Chain Reaction (PCR) is a fundamental laboratory technique that amplifies specific DNA sequences, creating millions of copies from a small initial sample. The PCR master mix is a pre-mixed solution containing all essential components for DNA amplification: DNA polymerase enzyme, primers, nucleotides (dNTPs), buffer, and magnesium ions. This optimized mixture ensures consistent, efficient amplification reactions for various applications including genetic research, diagnostics, and forensic analysis. Learn more about PCR optimization →