Serial Dilution Calculator for Research Labs: Easy, Accurate, and Free
Our free online serial dilution calculator makes it simple for researchers, PhD students, and lab professionals to generate precise serial dilution protocols for ELISA
How to Use the Serial Dilution Calculator
Step-by-step guide (great for students and beginners):
- Enter your final volume per well or tube (e.g., 100 µL, perfect for 96-well plates or microtubes).
- Select your serial dilution factor, such as 1:2 for doubling dilutions (or 1:X for any custom factor).
- Choose the number of dilutions/steps you want (e.g., 8 for a standard curve).
- Input your starting stock concentration and select the appropriate units (µg/mL, mg/mL, nM, etc.).
- Optionally, edit the diluent name (e.g., PBS, water, buffer).
- Click “Generate Table” to automatically produce a clear, stepwise, complete dilution table.
- Review the clear, stepwise dilution table, which shows how much diluent and sample to add at each stage.
- Download as Excel or PDF to print, record, or upload to your electronic lab notebook.
Want to understand in detail? Step by step guide, How to Perform Serial Dilution for ELISA
Looking for dilution calculator to calculate how to dilute a stock solution of known concentration